Haemocytometer

Haemocytometer

The counting of blood cells after proper dilution is known as
haemocytometry and the instrument used to count the blood cells is called
haemocytometer. Using haemocytometry method, red cells, platelets and eosinophils are often counted. Now-a-days it is also used for counting cells
of bacteria, yeast, or algae.

Haemocytometer – instruments description

A haemocytometer consists of a counting chamber, a coverglass for
the counting chamber and diluting pipettes. Many types of counting
chambers are available. Improved Neubauer and Fauchs Rosenthal are the
two most commonly used counting chambers in laboratories.
RBCs and WBCs in blood cannot be counted as such. The blood
has to be diluted in specific isotonic solutions. RBC, diluting fluid is called
Hayem’s solution. For WBC counting Turk’s solution or Toisson solution
can be used.

The total number of cells is expressed per mm3. The isotonic diluting
fluid keeps up the cells intact. In WBC counting, the solution will lyse the
red blood cells and the remaining nucleated WBCs are counted. Venous
blood is used in blood cells counting.

Normal Range of RBCs in human is as follows

Men : 4.5 – 5.9 million/mm3
Women : 4.1 – 5.1 million/mm3
At birth : 4.0 – 5.6 million/mm3
Normal Range of WBCs in human is as follows :
Adults : 4,500 – 11,000/mm3
Neonates : 10,000 – 25,000/mm3

Clinical significance :

1. Decrease in the number of circulating erythrocytes indicates anaemia.
2. An increased number of erythrocytes indicates the possibility of
polycythemia.
3. An increase in WBC count for a transient period indicates bacterial
infection.
4. Progressive increase in abnormal WBC count indicates the possibility of
leukemia.

Related Topics in Zoology:

Bio Zoology All Important Topics

  1. Livestock and Management
  2. Important cattle breeds and their characteristics
  3. Milch breeds (or) Dairy breeds
  4. Dual purpose breeds & Draught breeds
  5. Exotic breeds of cattle
  6. Common diseases and control in Cattle – Contagious diseases
  7. Non-contagious diseases – Cattle
  8. Techniques adopted in cattle breeding
  9. Poultry Breeds
  10. Farming methods
  11. Poultry byproducts
  12. Fish Pond
  13. Edible Fishes Of Tamilnadu
  14. Medical Lab Techniques – Stethoscope
  15. Sphygmomanometer
  16. Haemocytometer
  17. Urine Sugar analysis
  18. ECG Electrocardiogram
  19. Computed Tomography CT
  20. Endoscopy (Laproscopy) techniques , Artificial Pacemaker
  21. Autoanalyser

Unit 7. THEORIES OF EVOLUTION Topic List Zoology

  1. Theories Of Evolution Introduction
  2. Lamarck Laws
  3. Neo Lamarckism
  4. Darwinism
  5. Theory of Natural Selection
  6. Objections to Darwinism
  7. Modern concept of Natural Selection

Comments

  1. Atay ShiaJanuary 21, 2022 at 1:32 AM

    مفصلي رائع ، أنت تقدم أهم المعلومات حول فوائد الرمان . إنها أيضًا مفيدة جدًا ، لذا شكرًا جزيلاً على هذه المعلومات الرائعة.

    ReplyDelete

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