Basic techniques of plant tissue culture
Basic techniques of plant tissue culture Basic techniques of plant tissue culture Techniques of plant tissue culture Techniques of plant tissue culture are listed below:- 1. Culture vessels The culture vessels used for plant tissue studies includes Erylenmayer flask (conical flask), petri plates and culture tubes (25 x 150mm). 2. Culture medium The important media used for all purpose experiment are Murashige and Skoog medium (MS medium), Gamborg medium (B5 medium), White medium (W medium) and Nitsch medium. The culture medium is closed with cotton plug/ or aluminium foil sheet. The pH of the medium is adjusted to 5.8 (acidic range). 3. Sterilization Sterilization is the technique employed to get rid of the microbes such as bacteria and fungi in the culture medium and plant tissues. So, it is important to sterilize the culture medium and plant tissue. The culture medium can be sterilised by keeping it in an autoclave and maintaining the temperature of 121’C for 15 minutes. The pla...